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stx2 plus electrode world precision instruments  (World Precision Instruments)


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    Structured Review

    World Precision Instruments stx2 plus electrode world precision instruments
    Stx2 Plus Electrode World Precision Instruments, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 96/100, based on 1479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/stx2+plus+electrode+world+precision+instruments/Chopstick+Electrode+Set+for+EVOM2%2C+-+DISCONTINUED/pm40890487-352-51-53
    Average 96 stars, based on 1479 article reviews
    stx2 plus electrode world precision instruments - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Imaging:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Microscopy:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    DNA Purification:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Gel Extraction:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Cytotoxicity Assay:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Sequencing:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Cell Culture:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Knock-Out:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..

    Modification:

    Article Title: Basal IFN-λ2/3 expression mediates tight junction formation in human epithelial cells.
    Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models T84 cells ATCC Cat #CCL-248 Calu-3 cells ATCC Cat #HTB-55 HEK293T cells ATCC Cat #CRL-3216 Human ileum-derived organoids Triana et al, 2021 Recombinant DNA plentiCRISPR V2 blasti Addgene Cat #52961 pMDG.2 Addgene Cat #12259 psPAX Addgene Cat #12260 pLKO.1 neo Addgene Cat #13425 pLKO.1 puro Addgene Cat #8453 Antibodies Alpha-Tubulin Sigma Cat #T9026 β-actin Sigma Cat #A5441 Lamin-B1 Santa Cruz Cat #sc-374015 IRF3 Cell Signaling Cat #11904 T phospho-IRF3 NEB/Cell Signaling Cat #4947 ISG15 Santa Cruz Cat #166755 Claudin-2 Invitrogen Cat #51-6100 ZO-1 Invitrogen Cat #40-2200 ZO-1 Invitrogen Cat #33-9100 STAT1 BD Biosciences Cat #610115 phospho-STAT1 BD Biosciences Cat #612233 STING Cell Signaling Cat #13647 TBK1/NAK Cell Signaling Cat #3013S phospho-TBK1/NAK Cell Signaling Cat #5483S YAP/TAZ Santa Cruz Cat #sc-101199 YAP/TAZ Cell Signaling Cat #8418 phospho-YAP Cell Signaling Cat #4911S anti-mouse-IgG HRP Abcam Cat #ab6789 anti-rabbit-IgG HRP Abcam Cat #ab97051 anti-mouse-IgG IRDye® 680RD Licor Cat #926-68073 anti-rabbit-IgG IRDye® 800CW Licor Cat #926-32210 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number R https://www.rproject.org/ (R Core Team, 2023) — Prism GraphPad — Other 0.45-μm Syringe Filter Lab Unlimited Cat #W10462100 8-well chamber slide Ibidi Cat #80827-90 Transwell Inserts Thermo Fisher Cat #3415 48-well plate Fisher Cat #3548 EVOM3 Epithelial Volt/Ohm Meter World Precision Instruments — STX2-PLUS Electrode World Precision Instruments — Real-Time PCR System CFX Opus 96 Bio-Rad Cat #12011319 800TS Microplate Reader BioTek — Trans-Blot® TurboTM Transfer System Bio-Rad — ImageQuantTM LAS 4000 GE Healthcare — Odyssey M Imaging System Licor — ZEISS Celldiscoverer-7 (CD7) Widefield microscope ZEISS — Monarch® Genomic DNA Purification Kits NEB Cat #T3010S Monarch DNA Gel Extraction Kit NEB Cat #T1020S LDH-GloTM Cytotoxicity Assay Promega Cat #J2380 Sanger sequencing GENEWIZ (Azenta Life Sciences) — PureLinkTM Genomic DNA Mini Kit Thermo Fisher Cat #K182002 RNeasy Plus Mini Kit Qiagen Cat #74134 Illumina NovaSeq 6000 Illumina — Cell lines and cell culture Wild-type (WT) T84 (ATCC CCL-248) as well as T84 reporter and knock-out (KO) cells were cultured in a 50:50 mixture of Dulbecco’s Modified Eagle’s Medium (DMEM) and F12 (Gibco #11320033). ..



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